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The mixture was incubated at 37 C under anaerobic conditions for 24 h and then diluted with MRS broth to a concentration of 10^8 CFU/L using a spectrophotometer

Practical steps that can make a difference Apply a broad-spectrum sunscreen (SPF 30 or higher) every day to protect depigmented areas from sunburn and further pigment loss

O., & Cipolla-Neto, J

Although the F3 generation shows partial recovery of reproductive parameters, residual epigenetic alterations persist, suggesting that EED exposure creates lasting molecular signatures with potential multigenerational consequences

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